28 Background: Prevotella intermedia is a pathogenic bacteria in chronic periodontitis, which is associated with dental implant plaques. A stressed environment can trigger the expression of bacteria heat shock proteins. One of heat shock proteins expressed in P.intermedia is dnaJ. Objectives: Semiquantitative analysis of dnaJ mRNA Prevotella intermedia isolated from healthy dental implant and chronic periodontitis plaques. Methods: P.intermedia was isolated from healthy dental implant and chronic periodontitis plaques, then it was cultured during 6 days and 11 days. Expression of dnaJ mRNA was quantified by using Reverse-Transcription PCR, then the band of intensity expression of dnaJ mRNA was compared to the band of intensity expression of 16S rRNA. The expression was analized statistically. Result: The semiquantification result of intensity expression of dnaJ mRNA P.intermedia isolated from healthy dental implant [6 days;91,09%] and [11 days;88,42%], in isolated chronic periodontitis [6 days;87,03%] and [11 days;76,94%]. Conclusion: There are differences in the expression intensity of dnaJ mRNA P.intermedia between isolated healthy dental implants and chronic periodontitis, but it is not statistically significant. Key words:
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